Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells
نویسندگان
چکیده
منابع مشابه
Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells.
The purpose of this study was to compare the cytotoxicity of five one-step dentin-bonding agents on human dental pulp and odontoblast-like cells (MDPC-23). Photopolymerized and unpolymerized samples of these dentin-bonding agents were prepared and incubated with dental pulp or MDPC-23 cells. After 24 or 72 h of incubation, the number of unstained cells with trypan blue was counted. The staining...
متن کاملPolymerized bonding agents and the differentiation in vitro of human pulp cells into odontoblast-like cells.
OBJECTIVES Odontoblasts are highly differentiated post-mitotic cells, which under pathological conditions such as carious lesions and dental injuries may degenerate and be replaced by other pulp cells. We have recently shown that this physiological event can be reproduced in an in vitro assay system, but is highly modified by the presence of unpolymerized resinous monomers. Our hypothesis was t...
متن کاملCytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells.
OBJECTIVE The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells. STUDY DESIGN Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A co...
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This study was performed to standardize a dentin barrier test with the substitute and evaluate the cytotoxicity of one-step self-etching bonding agents. Each of the natural bovine dentin and polyurethane discs were 500-μm thick and were tested using a perfusion device. Following the treatment with 0.05% phenol on the natural bovine disc or three kinds of polyurethane discs-30, 40, and 50 pcf (p...
متن کاملCytotoxicity of low pH dentin-bonding agents in a dentin barrier test in vitro.
The reaction of three-dimensional cultures of pulp-derived cells in a dentin barrier test was recorded after exposure to All-Bond 2, Prime & Bond NT, Syntac SC, Syntac Classic, and Prompt L-Pop. The materials were applied on bovine dentin disks in a perfusion chamber, and the experiments were performed with (0.3 ml/h, 2 ml/h) and without perfusion of the pulpal part of the chamber. The cell rea...
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ژورنال
عنوان ژورنال: Journal of Oral Rehabilitation
سال: 2008
ISSN: 0305-182X,1365-2842
DOI: 10.1111/j.1365-2842.2008.01885.x